Anticorps Polyclonal de lapin anti-Phospho-JAK3 (Tyr980/981)

Phospho-JAK3 (Tyr980/981) Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29101-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 29101-1-AP (same clone as 29101-1-PBS)

  Non-treated and IL-4 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 29101-1-AP (Phospho-JAK3 (Tyr980/981) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin antibody as loading control. This data was developed using the same antibody clone with 29101-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29101-1-PBS cible Phospho-JAK3 (Tyr980/981) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Janus kinase 3 (a protein tyrosine kinase, leukocyte)
• Masse moléculaire calculée: 1124 aa, 125 kDa
• Poids moléculaire observé: 125 kDa
• Numéro d’acquisition GenBank: BC028068
• Symbole du gène: JAK3
• Identification du gène (NCBI): 3718
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

JAK3 belongs to the Janus kinase family of receptor-associated tyrosine kinases located in cytoplasm adjacent to the plasma membrane. Janus kinase family comprise four tyrosine (Tyr) kinases (JAK1, JAK2, JAK3 and Tyk2). In the cytoplasm, JAKs play a pivotal role in signal transduction via its association with type I and type II cytokine receptors, which include receptors for interleukins (ILs), interferons (IFNs), and hormones such as leptin. Interaction of a cytokine with its membrane-bound receptor complex triggers activation of receptor-bound JAKs, which phosphorylate the receptor on key cytoplasmic Tyr residues. These act as docking sites for SH2 domain-mediated interactions with STAT proteins. Receptor-bound STATs are then phosphorylated on conserved Tyr residues within their C terminal domains prior to dissociation from the receptor and formation of homo- or heterodimers. These translocate to the nucleus, bind target gene promoters, and stimulate target gene transcription. (PMID: 30948191)

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