Anticorps Monoclonal anti-Phospho-JNK (Thr183/Tyr185)

Phospho-JNK (Thr183/Tyr185) Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2H8F12

N° de cat : 60666-1-Ig

Synonymes

JNK (Thr183/Tyr185), Phospho-JNK, phospho-JNK(Thr183/Tyr185), phospho‐JNK/SAPK (Thr183/Tyr185), phospho-JNK1/2 (Thr183/Tyr185)

---

Galerie de données de validation

• 

  WB analysis using 60666-1-Ig

  Non-treated and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as a loading control.

• 

  WB analysis using 60666-1-Ig

  Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control.

• 

  WB analysis using 60666-1-Ig

  Non-treated and UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control.

• 

  WB analysis using 60666-1-Ig

  Non-treated and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control.

• 

  IHC staining of Jurkat using 60666-1-Ig

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HSC-T6, cellules NIH/3T3, cellules NIH/3T3 traitées au UV
• Résultats positifs en IHC: cellules Jurkat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

60666-1-Ig cible Phospho-JNK (Thr183/Tyr185) dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase 8
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 42 kDa, 50 kDa
• Numéro d’acquisition GenBank: NM_138982
• Symbole du gène: JNK
• Identification du gène (NCBI): 5599
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. The antibody can detect endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with JNK singly phosphorylated at Thr183.

Publications

Species	Application	Title
mouse	WB	Mater Today Bio; Proanthocyanidin capsules remodel the ROS microenvironment via regulating MAPK signaling for accelerating diabetic wound healing; Authors - Shaoyin Wei
mouse	WB	Int Immunopharmacol; DPHB inhibits osteoclastogenesis by suppressing NF-κB and MAPK signaling and alleviates inflammatory bone destruction; Authors - Caixia Liu
human	WB	Cell Rep; High-glucose-associated YTHDC1 lactylation reduces the sensitivity of bladder cancer to enfortumab vedotin therapy; Authors - Zhuo Xing

{{ptg:RelatedPrimaryAntibodies}}