Anticorps Monoclonal anti-Phospho-JNK (Thr183/Tyr185)

Phospho-JNK (Thr183/Tyr185) Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2H8F12

N° de cat : 60666-1-PBS

Synonymes

JNK, MAPK8, JNK 2, JNK 1, EC:2.7.11.24

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Galerie de données de validation

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  WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)

  Non-treated and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (HRP-60004) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

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  WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)

  Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

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  WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)

  Non-treated and UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

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  WB analysis using 60666-1-Ig (same clone as 60666-1-PBS)

  Non-treated and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (HRP-66095) antibody as a loading control. This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

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  IHC staining of Jurkat using 60666-1-Ig (same clone as 60666-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Jurkat cells slide using 60666-1-Ig (Phospho-JNK (Thr183/Tyr185) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60666-1-PBS in a different storage buffer formulation.

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Informations sur le produit

60666-1-PBS cible Phospho-JNK (Thr183/Tyr185) dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase 8
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 42 kDa, 50 kDa
• Numéro d’acquisition GenBank: NM_138982
• Symbole du gène: JNK
• Identification du gène (NCBI): 5599
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAPK8(Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. The JNK gene generates 10 forms of JNK through alternative splicing, and the protein encoded by the JNK gene has or does not have a COOH terminal, resulting in 46 kDa and 54 kDa proteins. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. The antibody can detect endogenous levels of p46 and p54 SAPK/JNK when phosphorylated at Thr183 and Tyr185. It will also react with JNK singly phosphorylated at Thr183.

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