Anticorps Recombinant de lapin anti-Phospho-JUN (Ser63)

Phospho-JUN (Ser63) Recombinant Antibody for WB, ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

240965B7

N° de cat : 84147-1-RR

Synonymes

c-Jun (Ser63), P-cJun, p-c-Jun, p-c-Jun (Ser63), p-c-Jun(Ser63)

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Galerie de données de validation

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  WB analysis using 84147-1-RR

  Non-treated NIH/3T3 cells, Anisomycin treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84147-1-RR (Phospho-JUN (Ser63) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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  ChIP experiment of PC-12 using 84147-1-RR

  Chromatin was prepared from PC-12 cells starved overnight and treated with Human β-Nerve Growth Factor (hβ-NGF) (50 ng/ml, 2 hr). Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of Phospho-JUN (Ser63) (84147-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3 traitées à l'anisomycine, cellules NIH/3T3 traitées au UV
• Résultats positifs en ChIP-qPCR: hβ-NGF (50 ng/ml, 2 hr) PC-12 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84147-1-RR cible Phospho-JUN (Ser63) dans les applications de WB, ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: jun oncogene
• Masse moléculaire calculée: 331 aa, 36 kDa
• Poids moléculaire observé: 40-48 kDa
• Numéro d’acquisition GenBank: BC068522
• Symbole du gène: JUN
• Identification du gène (NCBI): 3725
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis.The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. (PMID: 22180088, PMID: 17804415)

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