Anticorps Polyclonal de lapin anti-Phospho-JUN (Ser73)
Phospho-JUN (Ser73) Polyclonal Antibody for WB, IF/ICC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain, rat, souris et plus (1)
Applications
WB, IF/ICC, CoIP, ELISA
Conjugaison
Non conjugué
N° de cat : 28891-1-AP
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | cellules NIH/3T3 traitées au UV, |
Résultats positifs en IF/ICC | cellules NIH/3T3, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
WB | See 23 publications below |
IF | See 3 publications below |
CoIP | See 1 publications below |
Informations sur le produit
28891-1-AP cible Phospho-JUN (Ser73) dans les applications de WB, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris
Réactivité | Humain, rat, souris |
Réactivité citée | Humain, porc, souris |
Hôte / Isotype | Lapin / IgG |
Clonalité | Polyclonal |
Type | Anticorps |
Immunogène | Peptide |
Nom complet | jun oncogene |
Masse moléculaire calculée | 331 aa, 36 kDa |
Poids moléculaire observé | 38-45 kDa |
Numéro d’acquisition GenBank | BC068522 |
Symbole du gène | JUN |
Identification du gène (NCBI) | 3725 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par affinité contre l'antigène |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis (PMID: 22180088). JUN is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. JUN is a basic leucine zipper (bZIP) transcription factor that acts as homo- or heterodimer, binding to DNA and regulating gene transcription (PMID: 9732876). In additon, extracellular signals can induce post-translational modifications of JUN, resulting in altered transcriptional activity and target gene expression (PMID:8464713). More over, it has uncovered multiple layers of a complex regulatory scheme in which JUN is able to crosstalk, amplify and integrate different signals for tissue development and disease. Jun is predominantly nuclear, ubiquitinated Jun colocalizes with lysosomal proteins (PMID: 15469925). This antibody is raised against synthetic phosphopeptide corresponding to residues surrounding Ser73 of human JUN, which can detect the bands around 42-45 kDa.
Protocole
Product Specific Protocols | |
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WB protocol for Phospho-JUN (Ser73) antibody 28891-1-AP | Download protocol |
IF protocol for Phospho-JUN (Ser73) antibody 28891-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Small Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy | ||
J Cell Biol JNK regulates ciliogenesis through the interflagellar transport complex and actin networks | ||
J Cell Mol Med SIAH2 suppresses c-JUN pathway by promoting the polyubiquitination and degradation of HBx in hepatocellular carcinoma | ||
Biochem Pharmacol Activation of CXCL13/CXCR5 axis aggravates experimental autoimmune cystitis and interstitial cystitis/bladder pain syndrome. | ||
Int J Biol Sci RHOV promotes lung adenocarcinoma cell growth and metastasis through JNK/c-Jun pathway. | ||
Front Pharmacol Azithromycin Attenuates Bleomycin-Induced Pulmonary Fibrosis Partly by Inhibiting the Expression of LOX and LOXL-2. |