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Anticorps Recombinant de lapin anti-Phospho-JUN (Thr91/93)

Phospho-JUN (Thr91/93) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

241983H8

N° de cat : CL488-81667-2

Synonymes

JUN (Thr91/93), p JUN (Thr91/93), Phospho JUN (Thr91/93), Phospho-JUN, Phospho-JUN Thr91/93

Galerie de données de validation

Filter:
  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite® Plus 488-conjugated Phospho-JUN (Thr91/9 (CL488-81667-2)

    FC experiment of NIH/3T3 using CL488-81667-2

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with UV which intracellularly stained with 0.25 ug Coralite® Plus 488 Phospho-JUN (Thr91/93) Recombinant Antibody (CL488-81667-2, Clone:241983H8)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en FC (Intra)cellules NIH/3T3 traitées au UV,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-81667-2 cible Phospho-JUN (Thr91/93) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet jun oncogene
Masse moléculaire calculée 331 aa, 36 kDa
Poids moléculaire observé48 kDa
Numéro d’acquisition GenBankBC068522
Symbole du gène JUN
Identification du gène (NCBI) 3725
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

JUN is also named as c-Jun and AP1, belongs to the bZIP family and Jun subfamily. JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis.The phosphorylation of c-Jun by Plk3 immunoprecipitates was not altered by the pre-removal of JNK from the cell lysates. In addition, the effect of UV irradiation-induced phosphorylation of c-Jun and apoptosis were not significantly affected by knockdown of JNK mRNA. (PMID: 22180088, PMID: 17804415)

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