Anticorps Polyclonal de lapin anti-Phospho-LATS1 (Thr1079)

Phospho-LATS1 (Thr1079) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 28998-1-AP

Synonymes

h warts, LATS1, Phospho-LATS1 (Thr1079), WARTS, WARTS protein kinase, wts

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Galerie de données de validation

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  WB analysis using 28998-1-AP

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 28998-1-AP (Phospho-LATS1 (Thr1079) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 1 publications below

Informations sur le produit

28998-1-AP cible Phospho-LATS1 (Thr1079) dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: LATS, large tumor suppressor, homolog 1 (Drosophila)
• Masse moléculaire calculée: 15 kDa, 127 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC015665
• Symbole du gène: LATS1
• Identification du gène (NCBI): 9113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The large tumour suppressor 1 (LATS1) acting as a tumour suppressor is a serine/threonine kinase of the AGC kinase family and is found to be down-regulated in various types of human cancers. Recently, LATS1 has been identified as a central player of the emerging tumour suppressive Hippo signaling pathway. In this pathway, the MST1/2 kinase phosphorylates activates LATS1/2 which, together with its co-factor MOB1, subsequently phosphorylates and inhibits the YAP transcriptional factor by preventing them from translocating to the nucleus. The antibody also detects the phosphorylated at Thr1041 of the LATS2. (PMID: 32460168, PMID: 30219064,PMID: 31792377)

Publications

Species	Application	Title
human	WB	Int J Biol Sci; S100 Calcium Binding Protein A16 Promotes Cell Proliferation by triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to inhibit Hippo pathway in Glioma development; Authors - Yifang Hu
human	WB	Cell Mol Biol Lett; KIAA1429-mediated m6A modification of CHST11 promotes progression of diffuse large B-cell lymphoma by regulating Hippo-YAP pathway; Authors - Xiaomin Chen
		J Transl Med; Modulation of SRC by SNTB1 activates the Hippo-YAP pathway during colon adenocarcinoma metastasis; Authors - Zhengyan Chang
human	WB,IHC	IUBMB Life; Upstream transcription factor 1 suppresses laryngeal squamous cell carcinoma progression through transcriptional activation of junctional adhesion molecule 3; Authors - Yue Jia
human	WB	Biochem Biophys Res Commun; IGF2BP1 enhances the stability of SIK2 mRNA through m6A modification to promote non-small cell lung cancer progression; Authors - Yan Xu
human	WB	Exp Eye Res; Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGFβ Ameliorates high myopia-induced ocular hypertension; Authors - Qianwen Bu

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