Anticorps Polyclonal de lapin anti-Phospho-MAP2K4 (Ser257/Thr261)

Phospho-MAP2K4 (Ser257/Thr261) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 29250-1-AP

Synonymes

JNK activating kinase 1, JNKK, JNKK1, MAP kinase kinase 4, MAP2K4, MAPK/ERK kinase 4, MAPKK 4, MEK 4, MEK4, MKK4, Phospho-MAP2K4, Phospho-MAP2K4 (Ser257/Thr261), P-MAP2K4, PRKMK4, SAPK/ERK kinase 1, SEK1, SERK1

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Galerie de données de validation

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  WB analysis using 29250-1-AP

  Non-treated PC-3 cells and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29250-1-AP (Phospho-MAP2K4 (Ser257/Thr261) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules PC-3 traitées au PDGF,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

29250-1-AP cible Phospho-MAP2K4 (Ser257/Thr261) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase 4
• Masse moléculaire calculée: 399 aa, 44 kDa
• Poids moléculaire observé: 44-50 kDa
• Numéro d’acquisition GenBank: BC036032
• Symbole du gène: MAP2K4
• Identification du gène (NCBI): 6416
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mitogen-activated protein kinase kinase 4 (MAP2K4), also known as MAP kinase kinase 4 (MKK4), is a second-level kinase in the hierarchical mitogen-activated protein kinase (MAPK) pathway and is regulated by Ras. MAP2K4 has been found to be stably mutated in 3-5 % of cancers, such as ovarian, prostate, and liver cancers. MAP2K4 is activated by MEKK1 or ASK1/TAK1/MLK3. It is unique among all known MAP2Ks in its ability to phosphorylate two different MAPKs, p38 and JNK (c-Jun N-terminal kinase), respectively. (PMID: 24487067, PMID: 33341545)

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Differences in p38-STAT3-S100A11 signaling after the administration of aristolochic acid I and IVa may account for the disparity in their nephrotoxicity; Authors - Zhong Xian
human	WB	J Inorg Biochem; Approaches to selective and potent inhibition of glioblastoma by vanadyl complexes: Inducing mitotic catastrophe and methuosis; Authors - Sha Xu
mouse	WB	Bioorg Med Chem; Synthesis and biological evaluation of lappaconitine analogues as potential anti-neuroinflammatory agents by side chain modification and scaffold hopping strategy; Authors - Feng Xing
human	WB	Exp Cell Res; The aberrantly activated AURKB supports and complements the function of AURKA in CALR mutated cells through regulating the cell growth and differentiation; Authors - Xueting Hu

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