Anticorps Polyclonal de lapin anti-Phospho-TAK1 (Thr187)

Phospho-TAK1 (Thr187) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 28958-1-AP

Synonymes

MAP3K7, Phospho-MAP3K7/TAK1 (Thr187), TAK1, TGF beta activated kinase 1, TGF1a

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Galerie de données de validation

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  WB analysis using 28958-1-AP

  Non-treated HEK-293T and Calyculin A treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28958-1-AP (Phospho-TAK1 (Thr187) antibody) at dilution of 1:5000 incubated 4℃ overnight. The membrane was stripped and reblotted with GAPDH antibody as a loading control.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

28958-1-AP cible Phospho-TAK1 (Thr187) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase kinase 7
• Masse moléculaire calculée: 579 aa, 64 kDa
• Poids moléculaire observé: 75-85 kDa
• Numéro d’acquisition GenBank: BC017715
• Symbole du gène: TAK1
• Identification du gène (NCBI): 6885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily.It plays an important role in the cascades of cellular responses evoked by changes in the environment.It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825).It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Mol Cell Proteomics; Global Phosphoproteomics Unveils Kinase-regulated Networks in Systemic Lupus Erythematosus; Authors - Shuhui Meng
human	WB	Leukemia; Thrombin receptor activating peptide-6 decreases acute graft-versus-host disease through activating GPR15; Authors - Cong Liu
human	WB	Cell Mol Immunol; SMAD7 expression in CAR-T cells improves persistence and safety for solid tumors; Authors - Sixin Liang
human	WB	Cell Death Dis; CRLF1 bridges AKT and mTORC2 through SIN1 to inhibit pyroptosis and enhance chemo-resistance in ovarian cancer; Authors - Cong Xiang
mouse	WB	Acta Pharm Sin B; Free fatty acid receptor-4 regulates T-cell-mediated allogeneic reaction through activating an aryl hydrocarbon receptor pathway; Authors - Maxwell Duah

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