Anticorps Recombinant de lapin anti-Phospho-MEK1 (Thr286)

Phospho-MEK1 (Thr286) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241910G6

N° de cat : 80629-2-PBS

Synonymes

MAP2K1, MEK1, MAPKK 1, MAP2K 1, EC:2.7.12.2

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 80629-2-RR (same clone as 80629-2-PBS)

  Non-treated NIH/3T3 cells and nocodazole treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80629-2-RR (Phospho-MEK1 (Thr286) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control. This data was developed using the same antibody clone with 80629-2-PBS in a different storage buffer formulation.

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Informations sur le produit

80629-2-PBS cible Phospho-MEK1 (Thr286) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 40-45 kDa
• Numéro d’acquisition GenBank: BC139729
• Symbole du gène: MEK1
• Identification du gène (NCBI): 5604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 enzymatic activity is regulated by site-specific phosphorylation that can be activated with phosphorylation of Ser217/Ser221 by Raf kinase or suppressed by phosphorylation of Thr286 and Thr292 by CDK1 and CDK5 or Thr292 and Thr386 by ERK1/2. (PMID: 31972311, PMID: 17928366, PMID: 22177953)

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