Anticorps Monoclonal anti-Phospho-MEK1 (Thr386)

Phospho-MEK1 (Thr386) Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1G6A2

N° de cat : 68015-1-PBS

Synonymes

MEK1 (Thr386), p MEK1 , p MEK1 (Thr386), p MEK1 Thr386, Phospho MEK1

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Galerie de données de validation

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  WB analysis using 68015-1-Ig (same clone as 68015-1-PBS)

  Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 68015-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 68015-1-Ig (same clone as 68015-1-PBS)

  Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 68015-1-PBS in a different storage buffer formulation.

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  WB analysis using 68015-1-Ig (same clone as 68015-1-PBS)

  Non-treated HeLa cells, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68015-1-PBS in a different storage buffer formulation.

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  WB analysis using 68015-1-Ig (same clone as 68015-1-PBS)

  Non-treated HEK-293 cells and nocodazole treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 68015-1-PBS in a different storage buffer formulation.

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  WB analysis using 68015-1-Ig (same clone as 68015-1-PBS)

  Non-treated A431 cells and nocodazole treated A431 cells were subjected to SDS PAGE followed by western blot with 68015-1-Ig (Phospho-MEK1 (Thr386) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 68015-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68015-1-Ig (same clone as 68015-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-MEK1 (Thr386) antibody (68015-1-Ig, Clone: 1G6A2 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 68015-1-PBS in a different storage buffer formulation.

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  Cytometric bead array in cell lysate using MP50180-1

  Cytometric bead array in cell lysate using MP50180-1, Phospho-MEK1 (Thr386) Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67872-1-PBS. Detection antibody: 68015-1-PBS. Cell lysate: Non-treated HeLa and Calyculin A treated HeLa (30μg/well). Non-related target OAT Monoclonal Matched Antibody Pair (MP50109-1P) was served as control.

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Informations sur le produit

68015-1-PBS cible Phospho-MEK1 (Thr386) dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC139729
• Symbole du gène: MEK1
• Identification du gène (NCBI): 5604
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)

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