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Anticorps Recombinant de lapin anti-Phospho-MEK1 (Thr386)

Phospho-MEK1 (Thr386) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

6K5

N° de cat : CL647-81304

Synonymes

MEK1 (Thr386), p MEK1 , p MEK1 (Thr386), p MEK1 Thr386, Phospho MEK1

Galerie de données de validation

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  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 647-conjugated Phospho-MEK1 (Thr386 (CL647-81304)

    FC experiment of HeLa using CL647-81304

    1X10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-Mek1 (Thr386) Recombinant Antibody (CL647-81304, Clone:6K5)(red), or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en FC (Intra)cellules HeLa traitées à la λ phosphatase,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-81304 cible Phospho-MEK1 (Thr386) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet mitogen-activated protein kinase kinase 1
Masse moléculaire calculée 43 kDa
Poids moléculaire observé40-50 kDa
Numéro d’acquisition GenBankBC139729
Symbole du gène MEK1
Identification du gène (NCBI) 5604
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAP2K1 encodes MAPK1, also known as MEK1. MEK1 variants can enhance MEK1 expression and ERK1 phosphorylation that together lead to continuous activation of MEK/ERK signaling pathway. MEK1 bind directly to ERK2 through a region in the N terminus of MEK. In addition, a proline-rich (PR) regulatory sequence in MEK is also involved in MEK-ERK association and signal propagation. The coupling between MEK1 and ERK2 is enhanced through phosphorylation on S298 in the MEK1 PR region, whereas phosphorylation on MEK1 T292 releases the complex. MEK1 T292 is a substrate of ERK2, but the site is also phosphorylated at a basal level when ERK2 is inhibited, suggesting several regulators of this site . Although the S298 site in MEK2 has been conserved, it lacks the T292 phosphorylation site, and it is not a substrate of PAK1. (PMID: 31972311, PMID: 17928366, PMID: 22177953)

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