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Anticorps Recombinant de lapin anti-Phospho-MEK2 (Thr394)

Phospho-MEK2 (Thr394) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241669E6

N° de cat : 84556-1-RR

Synonymes

MEK2 (Thr394), Phospho MEK2 (Thr394), Phospho-MEK2, p-MEK2, p-MEK2 (Thr394)

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of NIH/3T3 cells using Phospho-MEK2 (Thr394) Recombinant antibody (84556-1-RR)

    WB analysis of NIH/3T3 using 84556-1-RR

    Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84556-1-RR (Phospho-MEK2 (Thr394) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Applications testées

Résultats positifs en WBcellules NIH/3T3, cellules NIH/3T3 traitées à la λ phosphatase

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

84556-1-RR cible Phospho-MEK2 (Thr394) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet mitogen-activated protein kinase kinase 2
Masse moléculaire calculée 44 kDa
Poids moléculaire observé44 kDa
Numéro d’acquisition GenBankBC018645
Symbole du gène MEK2
Identification du gène (NCBI) 5605
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Protein A purfication
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MEK2(MAPK/ERK kinase 2) is also named as MAP2K2, MKK2, PRKMK2 and belongs to the MAP kinase kinase subfamily. MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1). MEK1 and MEK2 are closely related, dual-specificity tyrosine/threonine protein kinases found in the Ras/Raf/MEK/ERK mitogen-activated protein kinase (MAPK) signaling pathway.

Protocole

Product Specific Protocols
WB protocol for Phospho-MEK2 (Thr394) antibody 84556-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Reyes (Verified Customer) (09-24-2025)

phospho-MEK2 (in green) worked in human brain FFPE tissue, marking spefically astrocytes in the TSC sample.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Human Brain Cortex
Phospho-MEK2 (Thr394) Antibody Immunofluorescence validation (1:100 dilution) in Human Brain Cortex (Cat no:84556-1-RR)
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