Anticorps Recombinant de lapin anti-Phospho-MEK6 (Ser207)

Phospho-MEK6 (Ser207) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250293G8

N° de cat : 80064-2-RR

Synonymes

MKK6 (Ser207), Phospho-MEK6, Phospho-MEK6(Ser207), Phospho-MKK3 (Ser189), Phospho-MKK3 (Ser189)/MKK6 (Ser207)

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Galerie de données de validation

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  WB analysis of HEK-293 using 80064-2-RR

  Non-treated HEK-293 cells and UV treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80064-2-RR (Phospho-MEK6 (Ser207) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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  WB analysis using 80064-2-RR

  Non-treated NIH/3T3 cells and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80064-2-RR (Phospho-MEK6 (Ser207) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: UV treated HEK-293 cells, cellules NIH/3T3 traitées au UV

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80064-2-RR cible Phospho-MEK6 (Ser207) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase 6
• Masse moléculaire calculée: 334 aa, 37 kDa
• Poids moléculaire observé: 38 kDa, 40kDa
• Numéro d’acquisition GenBank: BC012009
• Symbole du gène: MEK6
• Identification du gène (NCBI): 5608
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAPKK6 (Dual specificity mitogen activated protein kinase kinase 6), also known as MAP2K6 / MEK6 / MKK6, is a member of MAPKK protein kinase family. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase. MEK6 phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines or environmental stress. Phosphorylation of Ser207 and Thr211 by MAP3Ks is critical for MEK6 activation, and acetylation of these resides by VopA blocks MEK6 activity(PMID: 26648936). The antibody also recognizes the ser218 phosphorylation site of MEK3.

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