Anticorps Monoclonal anti-Phospho-MKK7 (Ser271/Thr275)

Phospho-MKK7 (Ser271/Thr275) Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG3

Réactivité testée

Humain

Applications

WB

Conjugaison

Non conjugué

CloneNo.

1B4B5

N° de cat : 68050-1-Ig

Synonymes

JNK activating kinase 2, JNK kinase 2, JNKK 2, Jnkk2, MAP kinase kinase 7, MAP2K7, MAPK/ERK kinase 7, MAPKK 7, MAPKK7, MEK 7, MEK7, MKK7, Phospho-MKK7 (Ser271/Thr275), PRKMK7

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Galerie de données de validation

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  WB analysis using 68050-1-Ig

  Non-treated and Calyculin A treated, λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 68050-1-Ig (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 68050-1-Ig

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 68050-1-Ig (Phospho-MKK7 (Ser271/Thr275) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées à la λ phosphatase, cellules HEK-293, cellules HEK-293 traitées à la calyculine A, cellules HeLa traitées à la calyculine A

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68050-1-Ig cible Phospho-MKK7 (Ser271/Thr275) dans les applications de WB et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG3
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase 7
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47-52 kDa
• Numéro d’acquisition GenBank: BC038295
• Symbole du gène: MAP2K7
• Identification du gène (NCBI): 5609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dual specificity mitogen-activated protein kinase kinase 7 (MKK7), also known as MEK7 or MAP2K7, is a member of mitogen-activated kinase kinase (MAP2K) subfamily, and a key activator of c-Jun N-terminal kinase (JNK) signaling, a pathway that regulates primarily stress and inflammatory responses. MKK7 activity can be increased by either MKK7-autophosphorylation or phosphorylation of the Ser and Thr residues of the S-X-A-K-T motifs in the Kinase domain by upstream MEKK1, MEKK2, or MLK3.(PMID: 32783966, PMID: 31579105)