Anticorps Polyclonal de lapin anti-Phospho-MOBKL1B (Thr12)

Phospho-MOBKL1B (Thr12) Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 29027-1-AP

Synonymes

MOBKL1B (Thr12), p MOBKL1B , p MOBKL1B (Thr12), p MOBKL1B Thr12, Phospho MOBKL1B

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Galerie de données de validation

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  WB analysis using 29027-1-AP

  Phosphatase inhibitor treated and λ phosphatase treated PC-12 cells were subjected to SDS PAGE followed by western blot with 29027-1-AP (Phospho-MOBKL1B (Thr12) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of U2OS using 29027-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated U2OS cells using Phospho-MOBKL1B (Thr12) antibody (29027-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules PC-12, λ phosphatase treated PC-12 cells
• Résultats positifs en IF/ICC: λ phosphatase treated U2OS cells,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

29027-1-AP cible Phospho-MOBKL1B (Thr12) dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: MOB1, Mps One Binder kinase activator-like 1B (yeast)
• Masse moléculaire calculée: 216 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC003398
• Symbole du gène: MOBKL1B
• Identification du gène (NCBI): 55233
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MOBKL1B, also known as MOB1B, belongs to the MOB1/phocein family. MOBKL1B binds to and regulate downstream targets such as the NDR-family protein kinases and LATS1 kinase. MOB1 protein is a key regulator of large tumor suppressor 1/2 (LATS1/2) kinases in the Hippo pathway. MOBKL1A and MOBKL1B are phosphorylated by MST1/MST2 kinases at Thr35 and Thr12, and MST1/MST2-catalyzed phosphorylation of MOBKL1A/MOBKL1B in intact cells is sufficient to substantially retard cell-cycle progression (PMID: 18328708).

Publications

Species	Application	Title
human,mouse	WB	J Cell Physiol; m6 A-induced TRIB3 regulates Hippo pathway through interacting with LATS1 to promote the progression of lung adenocarcinoma; Authors - Jiamei Wu

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