Anticorps Polyclonal de lapin anti-Phospho-MOBKL1B (Thr12)

Phospho-MOBKL1B (Thr12) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29027-1-PBS

Synonymes

MOBKL1B (Thr12), p MOBKL1B , p MOBKL1B (Thr12), p MOBKL1B Thr12, Phospho MOBKL1B

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Galerie de données de validation

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  WB analysis using 29027-1-AP (same clone as 29027-1-PBS)

  Phosphatase inhibitor treated and λ phosphatase treated PC-12 cells were subjected to SDS PAGE followed by western blot with 29027-1-AP (Phospho-MOBKL1B (Thr12) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 29027-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 29027-1-AP (same clone as 29027-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated U2OS cells using Phospho-MOBKL1B (Thr12) antibody (29027-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 29027-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29027-1-PBS cible Phospho-MOBKL1B (Thr12) dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: MOB1, Mps One Binder kinase activator-like 1B (yeast)
• Masse moléculaire calculée: 216 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC003398
• Symbole du gène: MOBKL1B
• Identification du gène (NCBI): 55233
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MOBKL1B, also known as MOB1B, belongs to the MOB1/phocein family. MOBKL1B binds to and regulate downstream targets such as the NDR-family protein kinases and LATS1 kinase. MOB1 protein is a key regulator of large tumor suppressor 1/2 (LATS1/2) kinases in the Hippo pathway. MOBKL1A and MOBKL1B are phosphorylated by MST1/MST2 kinases at Thr35 and Thr12, and MST1/MST2-catalyzed phosphorylation of MOBKL1A/MOBKL1B in intact cells is sufficient to substantially retard cell-cycle progression (PMID: 18328708).

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