Anticorps Recombinant de lapin anti-Phospho-MST1 (Thr183)/MST2 (Thr180)

Phospho-MST1 (Thr183)/MST2 (Thr180) Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(7)

CloneNo.

1P6

N° de cat : 80093-1-RR

Synonymes

MST1 (Thr183)/MST2 (Thr180), p MST1 (Thr183)/MST2 (Thr180), Phospho MST1 (Thr183), Phospho MST1 (Thr183)/MST2 (Thr180), Phospho MST2 (Thr180)

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Galerie de données de validation

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  WB analysis using 80093-1-RR

  Non-treated Ramos and Jurkat and Staurosporine treated Ramos and Jurkat cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (66240-1-Ig) antibody as a loading control.

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  WB analysis using 80093-1-RR

  Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (66240-1-Ig) antibody as a loading control.

• 

  WB analysis using 80093-1-RR

  Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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  FC experiment of HeLa using 80093-1-RR

  1X10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Anti-Human Phospho-MST1 (Thr183)/MST2 (Thr180) (80093-1-RR, Clone:1P6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HeLa, cellules HeLa traitées à la calyculine A, cellules Jurkat traitées à la staurosporine, cellules NIH/3T3 traitées à la calyculine A, cellules Ramos traitées à la staurosporine
• Résultats positifs en FC (Intra): cellules HeLa traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

80093-1-RR cible Phospho-MST1 (Thr183)/MST2 (Thr180) dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: serine/threonine kinase 4
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: BC005231
• Symbole du gène: MST1
• Identification du gène (NCBI): 6789
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.

Publications

Species	Application	Title
mouse	WB	Ecotoxicol Environ Saf; Role of the hippo signaling pathway in the extracellular matrix degradation of chondrocytes induced by fluoride exposure; Authors - Fang-fang Yu
mouse	WB	J Hazard Mater; Prenatal and postnatal exposure to polystyrene microplastics induces testis developmental disorder and affects male fertility in mice; Authors - Tianxin Zhao
mouse	WB	Nat Commun; KK-LC-1 as a therapeutic target to eliminate ALDH+ stem cells in triple negative breast cancer; Authors - Jiawen Bu
human	WB	Exp Eye Res; Targeting mechanics-induced trabecular meshwork dysfunction through YAP-TGFβ Ameliorates high myopia-induced ocular hypertension; Authors - Qianwen Bu
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Hippo pathway activated by circulating reactive oxygen species mediates cardiac diastolic dysfunction after acute kidney injury; Authors - Xiao Han
rat	WB	Cells; Activation of Yes-Associated Protein Is Indispensable for Transformation of Kidney Fibroblasts into Myofibroblasts during Repeated Administration of Cisplatin; Authors - Jia-Bin Yu

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