Anticorps Recombinant de lapin anti-Phospho-Myosin Light Chain 2 (Thr18/Ser19)

Phospho-Myosin Light Chain 2 (Thr18/Ser19) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250098F12

N° de cat : 85804-1-PBS

Synonymes

MYL12B, MYL9, P MYL12A, P MYL12B, P Myosin Light Chain 2 (Thr18/Ser19)

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Galerie de données de validation

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  WB analysis of HEK-293 using 85804-1-RR (same clone as 85804-1-PBS)

  Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85804-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 85804-1-RR (same clone as 85804-1-PBS)

  Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85804-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HSC-T6 using 85804-1-RR (same clone as 85804-1-PBS)

  Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 85804-1-RR (Phospho-Myosin Light Chain 2 (Thr18/Ser19) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85804-1-PBS in a different storage buffer formulation.

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Informations sur le produit

85804-1-PBS cible Phospho-Myosin Light Chain 2 (Thr18/Ser19) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin, light chain 12A, regulatory, non-sarcomeric
• Masse moléculaire calculée: 171 aa, 20 kDa
• Poids moléculaire observé: 19-20 kDa
• Numéro d’acquisition GenBank: BC016372
• Symbole du gène: MYL12A
• Identification du gène (NCBI): 10627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Myosin II is an actin-binding protein composed of MHC (myosin heavy chain), MLCs/MRLCs (myosin regulatory light chains) and ELCs (essential light chains). The Myosin Light Chains are divided into basic MLC (MLC I) and regulatory MLC (MLC II or Myosin Regulatory Light Chain, MRLC). MRLC can be classified into two major types: : class I MRLC found in muscles (MLC1, MLC2, MLC3, MYL4, MYL5, MYL6, MYL7, etc), and class II MRLCs present in non-muscle cells (such as MYL9, MYL12A, MYL12B). Phosphorylation of myosin II regulatory light chain (MRLC) is important for cell motility and cytokinesis in nonmuscle cells. Phosphorylation of MRLC at Ser19 (1P-MRLC) enhances myosin II activity, which is further enhanced by MRLC diphosphorylation at Thr18/Ser19 (2P-MRLC) in vitro .PMID: 23951055,PMID: 21126233
This antibody can recognize the phosphorylation of three highly homologous Myosin-regulated light chains: MYL12A, MYL12B and MYL9.

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