Anticorps Recombinant de lapin anti-Phospho-NF-κB p65 (Ser468)

Phospho-NF-κB p65 (Ser468) Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(230)

CloneNo.

6N1

N° de cat : 82335-1-RR

Synonymes

NFκB (Ser468), NF-κB (Ser468), NF-κB p65 (Ser468), p NF-κB, p NF-κB (Ser468)

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Galerie de données de validation

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  WB analysis using 82335-1-RR

  Non-treated and Calyculin A treated various cells were subjected to SDS PAGE followed by western blot with 82335-1-RR (Phospho-NF-κB p65 (Ser468) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Alpha Tubulin antibody (66031-1-Ig) and NF-κB p65 antibody (80979-1-RR) subsequently.

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  FC experiment of NIH/3T3 using 82335-1-RR

  1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-NF-κB p65 (Ser468) Recombinant antibody (82335-1-RR, Clone:6N1) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées à la calyculine A, cellules NIH/3T3 traitées à la calyculine A
• Résultats positifs en FC (Intra): cellules NIH/3T3 traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 221 publications below
• IHC: See 7 publications below
• IF: See 16 publications below

Informations sur le produit

82335-1-RR cible Phospho-NF-κB p65 (Ser468) dans les applications de WB, IHC, IF, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC011603
• Symbole du gène: NF-κB p65
• Identification du gène (NCBI): 5970
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.

Publications

Species	Application	Title
mouse	WB	Adv Sci (Weinh); Ccl2-Induced Regulatory T Cells Balance Inflammation Through Macrophage Polarization During Liver Reconstitution; Authors - Rui Wang
mouse	WB	Adv Sci (Weinh); Reprogramming Lung Redox Homeostasis by NIR Driven Ultra-Small Pd Loaded Covalent Organic Framework Inhibits NF-κB Pathway for Acute Lung Injury Immunotherapy; Authors - Doudou Lei
mouse	WB	Small; Polymer-Encapsulated Catalase for Targeted Redox Regulation in Acute Liver Injury; Authors - Feifei Li
	WB	Redox Biol; Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice; Authors - Boying Zhao
mouse		J Exp Clin Cancer Res; METTL3 recruiting M2-type immunosuppressed macrophages by targeting m6A-SNAIL-CXCL2 axis to promote colorectal cancer pulmonary metastasis; Authors - Peng Ouyang
mouse	WB	Carbohydr Polym; Structural characterizations and antiaging activities of hydrolyzed low-molecular-weight polysaccharides from Polygoni Multiflori Radix Praeparata; Authors - Wei Xu

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