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Anticorps Recombinant de lapin anti-Phospho-NF-κB p65 (Ser468)

Phospho-NF-κB p65 (Ser468) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

6N1

N° de cat : CL594-82335

Synonymes

NFκB (Ser468), NF-κB (Ser468), NF-κB p65 (Ser468), p NF-κB, p NF-κB (Ser468)

Galerie de données de validation

Filter:
  • Flow cytometry (FC) experiment of NIH/3T3 cells using CoraLite®594-conjugated Phospho-NF-κB p65 (Ser468) (CL594-82335)

    FC experiment of NIH/3T3 using CL594-82335

    1X10^6 NIH/3T3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Coralite®594 Phospho-NF-Κb P65 (Ser468) Recombinant Antibody (CL594-82335, Clone:6N1)(red), or 0.13 ug CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en FC (Intra)cellules NIH/3T3 traitées à la calyculine A,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-82335 cible Phospho-NF-κB p65 (Ser468) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Masse moléculaire calculée 65 kDa
Poids moléculaire observé75 kDa
Numéro d’acquisition GenBankBC011603
Symbole du gène NF-κB p65
Identification du gène (NCBI) 5970
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation.

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