Anticorps Polyclonal de lapin anti-Phospho-eNOS (Thr495)

Phospho-eNOS (Thr495) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 28939-1-AP

Synonymes

eNOS (Thr494), eNOS (Thr495), p eNOS , p eNOS (Thr495), p eNOS Thr495

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Galerie de données de validation

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  WB analysis using 28939-1-AP

  Non-treated, VEGF (HZ-1038), EGF and Cobalt Chloride treated HUVEC cells were subjected to SDS PAGE followed by western blot with 28939-1-AP (Phospho-NOS3 (Thr495) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Applications testées

• Résultats positifs en WB: cellules HUVEC traitées au VEGF, cellules HUVEC traitées à l'EGF, cellules HUVEC traitées au chlorure de cobalt

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

28939-1-AP cible Phospho-eNOS (Thr495) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nitric oxide synthase 3 (endothelial cell)
• Masse moléculaire calculée: 133 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC063294
• Symbole du gène: eNOS
• Identification du gène (NCBI): 4846
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Endothelial NOS (eNOS), also known as nitric oxide synthase 3 (NOS3), has a protective function in the cardiovascular system, which is attributed to NO production. Polymorphisms in NOS3 gene affects the susceptibility to several diseases such as hypertension, preeclampsia, diabetes mellitus, obesity, erectile dysfunction, and migraine. The sequence homology of eNOS protein in human and mouse is above 90%, and phosphorylation of threonine at 495 site in human corresponds to 494 site in mouse.

Publications

Species	Application	Title
human	WB	Hypertension; Statins Prevent the Deleterious Consequences of Placental Chemerin Upregulation in Preeclampsia; Authors - Lunbo Tan
mouse,human	WB	Commun Biol; Targeting GPR39 in structure-based drug discovery reduces Ang II-induced hypertension; Authors - Dongxu Hua
rat	WB	J Ethnopharmacol; Dahuang Zhechong pill attenuates hepatic sinusoidal capillarization in liver cirrhosis and hepatocellular carcinoma rat model via MK/integrin signaling pathway; Authors - Chuankui Fu
rat	WB	Drug Des Devel Ther; Clopidogrel-Induced Gastric Injury in Rats is Attenuated by Stable Gastric Pentadecapeptide BPC 157.; Authors - Hailu Wu
human	WB	J Oncol; Hax-1 Regulates Radiation-Induced Mitochondrial-Dependent Apoptosis of Uveal Melanoma Cells through PI3K/AKT/eNOS Pathway.; Authors - Sha Wang
human	WB	Cytotherapy; Porous gelatin microspheres implanted with adipose mesenchymal stromal cells promote angiogenesis via protein kinase B/endothelial nitric oxide synthase signaling pathway in bladder reconstruction; Authors - Jun Zhao

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