Anticorps Recombinant de lapin anti-Phospho-TrkA (Tyr496)/TrkB (Tyr516)

Phospho-TrkA (Tyr496)/TrkB (Tyr516) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241917B1

N° de cat : 84976-1-RR

Synonymes

Phospho-NTRK1, NTRK1 (Tyr496), Phospho-NTRK1 (Tyr496), Phospho-NTRK1-Tyr496, Phospho-TrkA

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Galerie de données de validation

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  WB analysis using 84976-1-RR

  Non-treated and pervanadate treated HeLa cells were subjected to SDS PAGE followed by western blot with 84976-1-RR (Phospho-NTRK1 (Tyr496) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: pervanadate treated HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84976-1-RR cible Phospho-TrkA (Tyr496)/TrkB (Tyr516) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: neurotrophic tyrosine kinase, receptor, type 1
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC062580
• Symbole du gène: NTRK1
• Identification du gène (NCBI): 4914
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The tyrosine kinase receptor TrkA (or neurotrophin receptor kinase 1, NTRK1) has been predominately studied for its role during the development of the nervous system. In developing neurons, TrkA activation upon binding of its ligands nerve growth factor (NGF) or neurotrophin-3 (NT-3) results in the stimulation of various tyrosine kinase-induced signaling pathways leading to neuronal outgrowth. (PMID: 32957504) This antibody recognizes the phosphorylation of TrkA at site 496, which is the phosphorylation of the TrkA-I(isoform 2) sequence at site 490.

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