Anticorps Recombinant de lapin anti-Phospho-P53 (Ser392)

Phospho-P53 (Ser392) Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

241575F6

N° de cat : 80077-2-RR

Synonymes

p P53, p P53 (Ser392), p P53 Ser392, P53 (Ser392), Phospho P53

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Galerie de données de validation

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  WB analysis of HEK-293 using 80077-2-RR

  Phosphatase inhibitor treated or λ phosphatase treated or untreated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80077-2-RR (Phospho-P53 (Ser392) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

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  FC experiment of HeLa using 80077-2-RR

  1x10^6 λ phosphatase treated HeLa cells were intracellularly stained with 0.2 ug Phospho-P53 (Ser392) Recombinant Antibody (80077-2-RR, Clone:241575F6)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9)(blue) and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated HeLa cells were intracellularly stained with 0.2 ug Phospho-P53 (Ser392) Recombinant Antibody (80077-2-RR, Clone:241575F6) and PE-conjugated Goat Anti-Rabbit IgG(black). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: λ phosphatase treated HEK-293 cells,
• Résultats positifs en FC (Intra): cellules HeLa traitées à la λ phosphatase,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80077-2-RR cible Phospho-P53 (Ser392) dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tumor protein p53
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC003596
• Symbole du gène: P53
• Identification du gène (NCBI): 7157
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P53 is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)

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