Anticorps Recombinant de lapin anti-Phospho-P53 (Ser46)

Phospho-P53 (Ser46) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250307A3

N° de cat : 80077-9-PBS

Synonymes

Phospho P53, Phospho P53 (Ser46), Phospho-P53, Phospho-P53(Ser46), Phospho-P53-Ser46

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Galerie de données de validation

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  WB analysis using 80077-9-RR (same clone as 80077-9-PBS)

  Non-treated HT-29 cells and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 80077-9-RR (Phospho-P53 (Ser46) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80077-9-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 80077-9-RR (same clone as 80077-9-PBS)

  Non-treated A431 cells and nocodazole treated A431 cells were subjected to SDS PAGE followed by western blot with 80077-9-RR (Phospho-P53 (Ser46) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80077-9-PBS in a different storage buffer formulation.

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Informations sur le produit

80077-9-PBS cible Phospho-P53 (Ser46) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tumor protein p53
• Masse moléculaire calculée: 44 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC003596
• Symbole du gène: P53
• Identification du gène (NCBI): 7157
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis. Homeodomain-interacting protein kinase 2 (HIPK2) Phosphorylates Ser46 off p53 and p38 can phosphorylate this site .

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