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Anticorps Monoclonal anti-Phospho-P53 (Ser46)

Phospho-P53 (Ser46) Monoclonal Antibody for FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D10A12

N° de cat : CL488-67900

Synonymes

Antigen NY CO 13, Cellular tumor antigen p53, FLJ92943, LFS1, P53, Phospho-P53 (Ser46), Phosphoprotein p53, TP53, TRP53, tumor protein p53, Tumor suppressor p53

Galerie de données de validation

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  • Flow cytometry (FC) experiment of HT-29 cells using CoraLite® Plus 488-conjugated Phospho-P53 (Ser46) (CL488-67900)

    FC experiment of HT-29 using CL488-67900

    1X10^6 HT-29 cells untreated (dashed lines) or treated with UV (red) were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human Phospho-P53 (Ser46) (CL488-67900, Clone:1D10A12), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en cytométrieUV treated HT-29 cells

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-67900 cible Phospho-P53 (Ser46) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet tumor protein p53
Masse moléculaire calculée 44 kDa
Poids moléculaire observé 53 kDa
Numéro d’acquisition GenBankBC003596
Symbole du gène TP53
Identification du gène (NCBI) 7157
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

P53 is a 53 kDa protein that is activated in response to alteration of normal cell homeostasis, including DNA damage, nutrient starvation, heat shock, virus infection, pH change, hypoxia, and oncogene activation. P53 maintains genetic stability by regulating different processes, such as cell-cycle arrest, DNA synthesis and repair, programmed cell death, and energy metabolism. In non-stressed conditions these proteins bind p53, ubiquitylate it and target it for degradation by the proteasome. In stressed conditions the function of the MdM2-MdM4 complex is blocked by phosphorylation, protein-binding events and/or enhanced degradation. (PMID: 19935675, PMID: 24379683)