Anticorps Polyclonal de lapin anti-Phospho-P62/SQSTM1 (Ser349)

Phospho-P62/SQSTM1 (Ser349) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 29503-1-AP

Synonymes

p p62 (Ser349), p p62 Ser349, p P62,SQSTM1, p P62,SQSTM1 (Ser349), p P62,SQSTM1 Ser349

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Galerie de données de validation

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  WB analysis using 29503-1-AP

  Non-treated HeLa cells and MG132 treated HeLa cells were subjected to SDS PAGE followed by western blot with 29503-1-AP (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 29503-1-AP

  Non-treated NIH/3T3 cells and MG132 treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29503-1-AP (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: MG132 treated HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IF: See 1 publications below

Informations sur le produit

29503-1-AP cible Phospho-P62/SQSTM1 (Ser349) dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: sequestosome 1
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC017222
• Symbole du gène: P62/SQSTM1
• Identification du gène (NCBI): 8878
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. It functions as a bridge between polyubiquitinated cargo and autophagosomes (PMID:16286508). SQSTM1 is at the cross-roads of several signaling pathways including Keap1-Nrf2 pathway , NFκB pathway , NFE2L2/NRF2 pathway , mTOR pathway and Wnt pathway. Phosphorylation and/or de-phosphorylation of p62-Ser349 may participate in the regulation of both selective autophagy and oxidative stress response (PMID: 33397898).

Publications

Species	Application	Title
mouse,human	WB	Cell Death Differ; SLC15A3 plays a crucial role in pulmonary fibrosis by regulating macrophage oxidative stress; Authors - Jun Luo
mouse	WB	Chin Med; NBR1-p62-Nrf2 mediates the anti-pulmonary fibrosis effects of protodioscin; Authors - Qian Zeng
rat	WB	J Med Chem; A Lysosome-Targeting hNEU1 Inhibitor Treats Myocardial Infarction: A Potential Therapeutic Breakthrough; Authors - Wen Zhou
mouse	IF	Cell Death Dis; TMEM232 is required for the formation of sperm flagellum and male fertility in mice; Authors - Xinying Cai
human	WB	J Biol Chem; Channel HCN4 mutation R666Q associated with sporadic arrhythmia decreases channel electrophysiological function and increases protein degradation; Authors - Hongrui Wang
human	WB	J Virol; The Porcine Cyclic GMP-AMP Synthase-STING Pathway Exerts an Unusual Antiviral Function Independent of Interferon and Autophagy; Authors - Sen Jiang

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