Anticorps Recombinant de lapin anti-Phospho-P62/SQSTM1 (Ser349)

Phospho-P62/SQSTM1 (Ser349) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250277E9

N° de cat : 80294-2-PBS

Synonymes

P62/SQSTM1 (Ser349), Phospho P62/SQSTM1 (Ser349), Phospho-P62 (Ser349), Phospho-P62/SQSTM1, Phospho-P62/SQSTM1(Ser349)

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Galerie de données de validation

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  WB analysis of HEK-293 using 80294-2-RR (same clone as 80294-2-PBS)

  Non-treated HEK-293 cells and sodium arsenite treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80294-2-RR (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80294-2-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 80294-2-RR (same clone as 80294-2-PBS)

  Non-treated NIH/3T3 cells and MG132 treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80294-2-RR (Phospho-P62/SQSTM1 (Ser349) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80294-2-PBS in a different storage buffer formulation.

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Informations sur le produit

80294-2-PBS cible Phospho-P62/SQSTM1 (Ser349) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: sequestosome 1
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 62 kDa
• Numéro d’acquisition GenBank: BC017222
• Symbole du gène: P62/SQSTM1
• Identification du gène (NCBI): 8878
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. It functions as a bridge between polyubiquitinated cargo and autophagosomes (PMID:16286508). SQSTM1 is at the cross-roads of several signaling pathways including Keap1-Nrf2 pathway , NFκB pathway , NFE2L2/NRF2 pathway , mTOR pathway and Wnt pathway. Phosphorylation and/or de-phosphorylation of p62-Ser349 may participate in the regulation of both selective autophagy and oxidative stress response (PMID: 33397898).

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