Anticorps Recombinant de lapin anti-Phospho-PAK1 (Ser144)/PAK2 (Ser141)

Phospho-PAK1 (Ser144)/PAK2 (Ser141) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242123D12

N° de cat : 85044-1-RR

Synonymes

PAK1 (Ser144), PAK1 (Ser144)/PAK2 (Ser141), PAK2 (Ser141), Phospho-PAK1, Phospho-PAK1 (Ser144)

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Galerie de données de validation

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  WB analysis using 85044-1-RR

  Non-treated RAW 264.7 cells and LPS treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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  WB analysis using 85044-1-RR

  Non-treated C6 cells and pervanadate treated C6 cells were subjected to SDS PAGE followed by western blot with 85044-1-RR (Phospho-PAK1 (Ser144)/PAK2 (Ser141) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: pervanadate treated C6 cells, cellules RAW 264.7 traitées au LPS

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85044-1-RR cible Phospho-PAK1 (Ser144)/PAK2 (Ser141) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: p21 protein (Cdc42/Rac)-activated kinase 1
• Masse moléculaire calculée: 553 aa, 62 kDa
• Poids moléculaire observé: 61 kDa
• Numéro d’acquisition GenBank: BC109299
• Symbole du gène: PAK1
• Identification du gène (NCBI): 5058
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The human PAK family is divided into the group I (PAK1 to PAK3) and group II (PAK4 to PAK6). Group I PAK share some domains that are not present in the group II members. In particular, the autoinhibitory domain (AID) is important for regulation of the kinase activity of the group I family members. Autophosphorylation at PAK1 Ser144, or at the equivalent sites for the other PAK, stabilizes the open conformation and sustains high kinase activity. Mutation of tyrosines 131 or 429 is associated with reduced dimerization and enhanced kinase activity. (PMID: 31748572)

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