Anticorps Polyclonal de lapin anti-Phospho-PDPK1 (Ser241)

Phospho-PDPK1 (Ser241) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 29241-1-AP

Synonymes

p PDPK1, p PDPK1 (Ser241), p PDPK1 Ser241, PDPK1 (Ser241), Phospho PDPK1

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Galerie de données de validation

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  WB analysis using 29241-1-AP

  Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29241-1-AP (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 29241-1-AP

  Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29241-1-AP (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:1000 incubated at 4℃ overnight.

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Applications testées

• Résultats positifs en WB: cellules PC-3 traitées au PDGF, cellules NIH/3T3 traitées au PDGF

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

29241-1-AP cible Phospho-PDPK1 (Ser241) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: 3-phosphoinositide dependent protein kinase-1
• Masse moléculaire calculée: 556 aa, 63 kDa
• Poids moléculaire observé: 58-68 kDa
• Numéro d’acquisition GenBank: BC012103
• Symbole du gène: PDPK1
• Identification du gène (NCBI): 5170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.

Publications

Species	Application	Title
human	WB	Cell Rep; A p85 isoform switch enhances PI3K activation on endosomes by a MAP4- and PI3P-dependent mechanism; Authors - Narendra Thapa

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