Anticorps Recombinant de lapin anti-Phospho-PDPK1 (Ser241)

Phospho-PDPK1 (Ser241) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250623A11

N° de cat : 85223-3-PBS

Synonymes

PDPK1, 3-phosphoinositide-dependent protein kinase 1, EC:2.7.11.1, hPDK1, PDK1

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 85223-3-RR (same clone as 85223-3-PBS)

  Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85223-3-RR (Phospho-PDPK1 (Ser241) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85223-3-PBS in a different storage buffer formulation.

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Informations sur le produit

85223-3-PBS cible Phospho-PDPK1 (Ser241) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: 3-phosphoinositide dependent protein kinase-1
• Masse moléculaire calculée: 556 aa, 63 kDa
• Poids moléculaire observé: 58-68 kDa
• Numéro d’acquisition GenBank: BC012103
• Symbole du gène: PDPK1
• Identification du gène (NCBI): 5170
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

3-Phosphoinositide dependent protein kinase-1 (PDK1 or PDPK1) is a serine-threonine kinase belonging to AGC kinase family. PDK1 plays a critical role in establishing ACD (Asymmetric cell division) in the epithelium (PMID: 27184845). The kinase activity of PDK1 depends on phosphatidyl inositol 3-kinase (PI3K), a key intermediate in signaling pathways including those from growth factor receptors and adhesion molecules. Substrates of PDK1, including AKT and the protein kinase C (PKC) isozymes, regulate a number of essential cell functions (PMID: 20027184). PDPK1 has 5 isoforms produced by alternative splicing of 48~63 kDa and is detected as 60-63 kDa.

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