Anticorps Recombinant de lapin anti-Phospho-PERK/EIF2AK3 (Ser719)

Phospho-PERK/EIF2AK3 (Ser719) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

243165A4

N° de cat : 81251-2-RR

Synonymes

P EIF2AK3 (Ser719), P PERK/EIF2AK3 (Ser719), P-EIF2AK3 (Ser719), Phos EIF2AK3 (Ser719), Phos PERK/EIF2AK3 (Ser719)

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Galerie de données de validation

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  WB analysis using 81251-2-RR

  Non-treated NIH/3T3 cells, phosphatase inhibitor treated and λ phosphatase NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81251-2-RR (Phospho-PERK/EIF2AK3 (Ser719) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as loading control.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules NIH/3T3 traitées à la λ phosphatase

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81251-2-RR cible Phospho-PERK/EIF2AK3 (Ser719) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: eukaryotic translation initiation factor 2-alpha kinase 3
• Masse moléculaire calculée: 1116 aa, 125 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC126354
• Symbole du gène: PERK
• Identification du gène (NCBI): 9451
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

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