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Anticorps Recombinant de lapin anti-Phospho-PERK/EIF2AK3 (Thr982)

Phospho-PERK/EIF2AK3 (Thr982) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4E16

N° de cat : CL488-82534

Synonymes

PERK/EIF2AK3 (Thr982), Phospho PERK/EIF2AK3, Phospho PERK/EIF2AK3 (Thr982), Phospho-PERK/EIF2AK3, Phospho-PERK/EIF2AK3 Thr982

Galerie de données de validation

Filter:
  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite® Plus 488-conjugated Phospho-PERK/EIF2AK3 (CL488-82534)

    FC experiment of HEK-293 using CL488-82534

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.25 ug Coralite® Plus 488 Phospho-PERK/EIF2AK3 (Thr982) Recombinant Antibody (CL488-82534, Clone:4E16)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en FC (Intra)cellules HEK-293 traitées à la calyculine A,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-82534 cible Phospho-PERK/EIF2AK3 (Thr982) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet eukaryotic translation initiation factor 2-alpha kinase 3
Masse moléculaire calculée 1116 aa, 125 kDa
Poids moléculaire observé180 kDa
Numéro d’acquisition GenBankBC126354
Symbole du gène PERK
Identification du gène (NCBI) 9451
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

EIF2AK3 encodes the protein kinase RNA-like ER kinase (PERK), a key regulator of the unfolded protein response (UPR) in response to ER stress. Under ER stress conditions, activation of PERK is triggered by the dissociation of glucose-regulated protein (GRP) 78 (also known as BiP) from its luminal domain, followed by oligomerization and autophosphorylation. Phosphorylated PERK subsequently phosphorylates eukaryotic translation initiation factor 2 alpha (eif2α), to attenuate global protein translation and reduce incoming ER protein load via upregulated ER chaperone expression. (PMID: 35922637, PMID: 32029570)

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