Anticorps Recombinant de lapin anti-Phospho-PKC Alpha (Ser657)

Phospho-PKC Alpha (Ser657) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250276F2

N° de cat : 83840-7-PBS

Synonymes

p PKC α (Ser 657), Phospho PKC Alpha, Phospho-PKC Alpha, Phospho-PKC Alpha(Ser657), Phospho-PKC Alpha-Ser657

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Galerie de données de validation

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  WB analysis using 83840-7-RR (same clone as 83840-7-PBS)

  Non-treated NIH/3T3 cells and PMA treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83840-7-RR (Phospho-PKC Alpha (Ser657) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 83840-7-PBS in a different storage buffer formulation.

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  WB analysis using 83840-7-RR (same clone as 83840-7-PBS)

  Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 83840-7-RR (Phospho-PKC Alpha (Ser657) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 83840-7-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 83840-7-RR (same clone as 83840-7-PBS)

  Non-treated HSC-T6 cells and Calyculin A treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 83840-7-RR (Phospho-PKC Alpha (Ser657) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 83840-7-PBS in a different storage buffer formulation.

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Informations sur le produit

83840-7-PBS cible Phospho-PKC Alpha (Ser657) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein kinase C, alpha
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: AK055431
• Symbole du gène: PKC Alpha
• Identification du gène (NCBI): 5578
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)

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