Anticorps Recombinant de lapin anti-Phospho-PKC Alpha (Thr638)

Phospho-PKC Alpha (Thr638) Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

241844A10

N° de cat : 81575-2-PBS

Synonymes

Phospho-PKC, Phospho-PKC Alpha, Phospho-PKC Alpha Thr638, Phospho-PKC beta (Thr641), Phospho-PKC α

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Galerie de données de validation

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  WB analysis using 81575-2-RR (same clone as 81575-2-PBS)

  Non-treated THP-1 cells, phosphatase inhibitor treated THP-1 cells and λ phosphatase treated THP-1 cells were subjected to SDS PAGE followed by western blot with 81575-2-RR (Phospho-PKC Alpha (Thr638) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81575-2-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 81575-2-RR (same clone as 81575-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated THP-1 cells using Phospho-PKC Alpha (Thr638) antibody (81575-2-RR, Clone: 241844A10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 81575-2-PBS in a different storage buffer formulation.

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  FC experiment of THP-1 using 81575-2-RR (same clone as 81575-2-PBS)

  1X10^6 THP-1 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.06 ug Phospho-PKC Alpha (Thr638) Recombinant antibody (81575-2-RR, Clone:241844A10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81575-2-PBS in a different storage buffer formulation.

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Informations sur le produit

81575-2-PBS cible Phospho-PKC Alpha (Thr638) dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein kinase C, alpha
• Masse moléculaire calculée: 77 kDa
• Poids moléculaire observé: 77 kDa
• Numéro d’acquisition GenBank: AK055431
• Symbole du gène: PKC Alpha
• Identification du gène (NCBI): 5578
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016)

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