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Anticorps Polyclonal de lapin anti-Phospho-PKN1 (Thr774)

Phospho-PKN1 (Thr774) Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29107-1-PBS

Synonymes

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-PKN1 (Thr774) Polyclonal antibody (29107-1-AP)

    WB analysis using 29107-1-AP (same clone as 29107-1-PBS)

    Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29107-1-AP (Phospho-PKN1 (Thr774) antibody) at dilution of 1:5000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 29107-1-PBS in a different storage buffer formulation.

Informations sur le produit

29107-1-PBS cible Phospho-PKN1 (Thr774) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet protein kinase N1
Masse moléculaire calculée 942 aa, 104 kDa
Poids moléculaire observé 130 kDa
Numéro d’acquisition GenBankBC040061
Symbole du gène PKN1
Identification du gène (NCBI) 5585
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PKN1 is a stress-responsive kinase and a member of the protein kinase novel (PKN) family also known as protein kinase C-related kinases (PRKs). PKN1 acting downstream of small GTP-binding proteins of the Rho/Rac family. The interaction of Rho with PKN1 induces a conformational change in PKN1 leading to activation loop phosphorylation by PDK1 on Thr774 in the case of human PKN1 (Thr778 in mouse/rat) which is necessary for the catalytic activation of PKN1/2 and critical for the stability of the protein. (PMID: 29045568, PMID: 21037231)

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