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Anticorps Polyclonal de lapin anti-Phospho-PLCG1 (Ser1248)

Phospho-PLCG1 (Ser1248) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29676-1-PBS

Synonymes

p PLCG1, p PLCG1 (Ser1248), p PLCG1 Ser1248, Phospho PLCG1, Phospho PLCG1 (Ser1248)

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using Phospho-PLCG1 (Ser1248) Polyclonal antibody (29676-1-AP)

    WB analysis using 29676-1-AP (same clone as 29676-1-PBS)

    Non-treated and Calyculin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 29676-1-AP (Phospho-PLCG1 (Ser1248) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29676-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Phospho-PLCG1 (Ser1248) Polyclonal antibody (29676-1-AP)

    IF Staining of HeLa using 29676-1-AP (same clone as 29676-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using Phospho-PLCG1 (Ser1248) antibody (29676-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29676-1-PBS in a different storage buffer formulation.

Informations sur le produit

29676-1-PBS cible Phospho-PLCG1 (Ser1248) dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène Peptide
Nom complet phospholipase C, gamma 1
Poids moléculaire observé145 kDa
Numéro d’acquisition GenBankBC144136
Symbole du gène PLCG1
Identification du gène (NCBI) 5335
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phosphoinositide phospholipase C-gamma-1 (PLCG1) which belongs to the phosphoinositide-specific phospholipase C (PLC) family, is activated by many extracellular stimuli including hormones, neurotransmitters, and growth factors and modulates several cellular and physiological functions necessary for tumorigeneses such as cell survival, migration, invasion, and angiogenesis by generating inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) via hydrolysis of phosphatidylinositol 4,5-biphosphate (PIP2) (PMID: 9242915). Phosphorylation is one of the key mechanisms that regulate the activity of PLC. PLCγ is activated by both receptor and non-receptor tyrosine kinases (PMID: 2472218). PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783, and 1248 (PMID: 1708307). It has also been shown that PKA-mediated phosphorylation at Ser1248 is inhibitory to PLCγ1 tyrosine phosphorylation and phospholipase activity in CD3-treated Jurkat cells (PMID: 1370476), suggesting that Ser1248 may be an allosteric regulator of PLCγ1 activity.

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