Anticorps Polyclonal de lapin anti-Phospho-PLCG2 (Tyr1217)

Phospho-PLCG2 (Tyr1217) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 29568-1-AP

Synonymes

Phospholipase C gamma 2, Phospholipase C IV, Phospho-PLCG2 (Tyr1217), PLC gamma 2, PLC IV, PLCG2

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Galerie de données de validation

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  WB analysis of Raji using 29568-1-AP

  Non-treated Raji cells and pervanadate treated Raji cells were subjected to SDS PAGE followed by western blot with 29568-1-AP (Phospho-PLCG2 (Tyr1217) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as loading control.

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Applications testées

• Résultats positifs en WB: pervanadate treated Raji cells,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

29568-1-AP cible Phospho-PLCG2 (Tyr1217) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: phospholipase C, gamma 2 (phosphatidylinositol-specific)
• Masse moléculaire calculée: 148 kDa
• Poids moléculaire observé: 148-150 kDa
• Numéro d’acquisition GenBank: BC007565
• Symbole du gène: PLCG2
• Identification du gène (NCBI): 5336
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Phospholipase C gamma 2 (PLCG2) belongs to the family of phospholipase C-gamma, which is the downstream molecule of the CSF1R. Previous studies have demonstrated that PLCG2 was required for CSF1-induced differentiation of monocytes, microglia, immune, inflammatory responses. PLCG2 phosphorylation leads to an increase in production of the cellular signaling molecules, diacylglycerol (DAG), and inositol 1,4,5 trisphosphate (IP3), which further promotes a wide range of downstream signals, including the phosphorylation of protein kinase C (PKC) family members, such as PKC epsilon (PKCε). (PMID: 32522286)

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