Anticorps Recombinant de lapin anti-Phospho-PLCG2 (Tyr1217)

Phospho-PLCG2 (Tyr1217) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

240631F2

N° de cat : 84456-1-PBS

Synonymes

PLCG2, Phospholipase C IV, Phospholipase C gamma 2, Phosphoinositide phospholipase C-gamma-2, EC:3.1.4.11

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Galerie de données de validation

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  WB analysis using 84456-1-RR (same clone as 84456-1-PBS)

  Non-treated and pervanadate treated Ramos cells were subjected to SDS PAGE followed by western blot with 84456-1-RR (Phospho-PLCG2 (Tyr1217) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 84456-1-PBS in a different storage buffer formulation.

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Informations sur le produit

84456-1-PBS cible Phospho-PLCG2 (Tyr1217) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: phospholipase C, gamma 2 (phosphatidylinositol-specific)
• Masse moléculaire calculée: 148 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC007565
• Symbole du gène: PLCG2
• Identification du gène (NCBI): 5336
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Phospholipase C gamma 2 (PLCG2) belongs to the family of phospholipase C-gamma, which is the downstream molecule of the CSF1R. Previous studies have demonstrated that PLCG2 was required for CSF1-induced differentiation of monocytes, microglia, immune, inflammatory responses. PLCG2 phosphorylation leads to an increase in production of the cellular signaling molecules, diacylglycerol (DAG), and inositol 1,4,5 trisphosphate (IP3), which further promotes a wide range of downstream signals, including the phosphorylation of protein kinase C (PKC) family members, such as PKC epsilon (PKCε). (PMID: 32522286)

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