Anticorps Polyclonal de lapin anti-Phospho-POLR2A (Ser2)

Phospho-POLR2A (Ser2) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 30888-1-AP

Synonymes

hRPB220, hsRPB1, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2

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Galerie de données de validation

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  WB analysis using 30888-1-AP

  Phosphatase inhibitor treated C2C12 cells, λ phosphatase treated C2C12 cells, and non-treated C2C12 cells were subjected to SDS PAGE followed by western blot with 30888-1-AP (Phospho-POLR2A (Ser2) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: cellules C2C12, λ phosphatase treated C2C12 cells

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30888-1-AP cible Phospho-POLR2A (Ser2) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Masse moléculaire calculée: 1970 aa, 217 kDa
• Poids moléculaire observé: 250 kDa
• Numéro d’acquisition GenBank: BC137231
• Symbole du gène: POLR2A
• Identification du gène (NCBI): 5430
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNA-directed RNA polymerase II subunit RPB1 is the catalytic core component of DNA-dependent RNA polymerase II (Pol II). So that the information encoded in DNA can be used, RNA polymerase II makes copies of specific genes. To ensure that RNA polymerase II copies the correct genes at the correct time, a group of regulatory proteins are needed to control its activity. Many of these proteins interact with RNA polymerase II at a region known as the C-terminal domain, or CTD for short. For example, before RNA polymerase can make a full copy of a gene, a small molecule called a phosphate group must first be added to CTD at specific units known as Ser2. (PMID: 31385803)

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