Anticorps Polyclonal de lapin anti-Phospho-PRAS40 (Thr246)

Phospho-PRAS40 (Thr246) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 29072-1-AP

Synonymes

AKT1S1, AKTS1, Lobe, Phospho-PRAS40 (Thr246), PRAS40, Proline rich AKT1 substrate 1

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Galerie de données de validation

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  WB analysis using 29072-1-AP

  Non-treated MCF-7 cells and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 29072-1-AP (Phospho-PRAS40 (Thr246) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 29072-1-AP

  Non-treated PC-3 cells and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29072-1-AP (Phospho-PRAS40 (Thr246) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7 traitées à l'IGF-1, cellules PC-3 traitées à la calyculine A

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

29072-1-AP cible Phospho-PRAS40 (Thr246) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: AKT1 substrate 1 (proline-rich)
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC051844
• Symbole du gène: AKT1S1
• Identification du gène (NCBI): 84335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis, as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB/Akt), nuclear factor kappa-B(NF-κB), proto-oncogene serine/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2). PGK1 phosphorylated PRAS40 at Threonine 246, which could be inhibited by blocking the interaction. PRAS40 can be phosphorylated by p-AKT at Thr246 site and promote the signal downstream to the mTOR signaling pathway. (PMID: 35058442, PMID: 32855714)