Anticorps Recombinant de lapin anti-Phospho-PRAS40 (Thr246)

Phospho-PRAS40 (Thr246) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250305A2

N° de cat : 80565-2-PBS

Synonymes

p PRAS40 (Thr246), Phospho PRAS40 (Thr246), Phospho-PRAS40, Phospho-PRAS40(Thr246), Phospho-PRAS40-Thr246

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Galerie de données de validation

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  WB analysis using 80565-2-RR (same clone as 80565-2-PBS)

  Non-treated HeLa cells and Insulin treated HeLa cells were subjected to SDS PAGE followed by western blot with 80565-2-RR (Phospho-PRAS40 (Thr246) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80565-2-PBS in a different storage buffer formulation.

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Informations sur le produit

80565-2-PBS cible Phospho-PRAS40 (Thr246) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: AKT1 substrate 1 (proline-rich)
• Masse moléculaire calculée: 27 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: BC051844
• Symbole du gène: PRAS40
• Identification du gène (NCBI): 84335
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PRAS40 is expressed in a variety of tissues in vivo and has multiple phosphorylation sites, which its activity is closely related to phosphorylation. Studies have shown that PRAS40 is involved in regulating cell growth, cell apoptosis, oxidative stress, autophagy and angiogenesis, as well as various of signalling pathways such as mammalian target of mammalian target rapamycin (mTOR), protein kinase B (PKB/Akt), nuclear factor kappa-B(NF-κB), proto-oncogene serine/threonine-protein kinase PIM-1(PIM1) and pyruvate kinase M2 (PKM2). PGK1 phosphorylated PRAS40 at Threonine 246, which could be inhibited by blocking the interaction. PRAS40 can be phosphorylated by p-AKT at Thr246 site and promote the signal downstream to the mTOR signaling pathway. (PMID: 35058442, PMID: 32855714)

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