Anticorps Recombinant de lapin anti-Phospho-PRKD1 (Ser916)

Phospho-PRKD1 (Ser916) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

241786A11

N° de cat : 80080-2-RR

Synonymes

phospho-PKD, Phospho-PKD (Ser916), Phospho-PKD/PKCμ, Phospho-PKD/PKCμ (Ser916), Phospho-PKD/PKCμ Ser916

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Galerie de données de validation

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  WB analysis using 80080-2-RR

  Non-treated NIH/3T3 cells, phosphatase inhibitor treated NIH/3T3 cells and λ phosphatase treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80080-2-RR (Phospho-PRKD1 (Ser916) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules NIH/3T3 traitées à la λ phosphatase

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80080-2-RR cible Phospho-PRKD1 (Ser916) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein kinase D1
• Masse moléculaire calculée: 102 kDa
• Poids moléculaire observé: 115 kDa
• Numéro d’acquisition GenBank: NM_001330069
• Symbole du gène: PRKD1
• Identification du gène (NCBI): 5587
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase D1 (PRKD1), also named as PKD1 and PKCμ, is comprised of two cysteine-rich domains and a pleckstrin homology (PH) domain. PKD1 is involved in cellular processes including protein secretion, proliferation, cytoskeletal reorganization, Golgi function, immune function and apoptosis. It is widely expressed in thyroid, brain, heart, lung and other tissues. PKCs have been shown to regulate PKD1 activation. It has been reported that ser 916 is a PKD1 autophosphorylation site. PKD1 can be activated by growth factors, oxidative stress, thrombin, bioactive lipids, cross-linking of B- and T-cell receptors and some G-protein coupled receptors (GPCR). PKD1 is located mainly in the cytoplasm in unstimulated cells, while PKD1 migrates to the membrane in activated cells. (PMID: 17306383, 24806360, 30101477, 21696630)

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