Anticorps Recombinant de lapin anti-Phospho-RASGRF1 (Ser927)

Phospho-RASGRF1 (Ser927) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5K3

N° de cat : 82309-1-PBS

Synonymes

RASGRF1, GRF55, GRF1, GNRP, CDC25L

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Galerie de données de validation

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  WB analysis of HeLa using 82309-1-RR (same clone as 82309-1-PBS)

  Non-treated and Forskolin treated HeLa cells were subjected to SDS PAGE followed by western blot with 82309-1-RR (Phospho-RASGRF1 (Ser927) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR (66888-1-Ig) as loading control. This data was developed using the same antibody clone with 82309-1-PBS in a different storage buffer formulation.

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Informations sur le produit

82309-1-PBS cible Phospho-RASGRF1 (Ser927) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Ras protein-specific guanine nucleotide-releasing factor 1
• Masse moléculaire calculée: 134 kDa
• Poids moléculaire observé: 145 kDa
• Numéro d’acquisition GenBank: BC040275
• Symbole du gène: RASGRF1
• Identification du gène (NCBI): 5923
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The RASGRF1 exchange factor, which is also known as CDC25 in Saccharomyces cerevisiae, promotes the exchange of Ras-bound GDP by GTP, is known to play central roles in pathways of cellular growth and differentiation. The serine residue at position 916 in mouse Ras-GRF1 is a PKA phosphorylation site, and the corresponding human residue is serine 927. It's reported that phosphorylation of serine 916 is necessary but not sufficient for maximal activation of mouse RASGRF1. The calculated MW of non-phosphorylated RASGRF1 is 145 kDa. 82309-1-RR can detect a band around 145 kDa, and can detect the increasing phosphorylation level after Forskolin treated.(PMID:15853814, 10601308)

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