Anticorps Monoclonal anti-Phospho-RIPK1 (Ser161)

Phospho-RIPK1 (Ser161) Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B2G1

N° de cat : CL488-66854

Synonymes

RIPK1, RIP, Receptor-interacting serine/threonine-protein kinase 1, Receptor-interacting protein 1, Receptor interacting serine/threonine protein kinase 1

---

Galerie de données de validation

• 

  IF Staining of U2OS using CL488-66854

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using CoraLite® Plus 488 Phospho-RIPK1 (Ser161) antibody (CL488-66854, Clone: 1B2G1 ) at dilution of 1:100.

• 

  IF Staining of HeLa using CL488-66854

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using CoraLite® Plus 488 Phospho-RIPK1 (Ser161) antibody (CL488-66854, Clone: 1B2G1 ) at dilution of 1:200.

• 

  FC experiment of HEK-293T using CL488-66854

  1X10^6 HEK-293T cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.13 ug CoraLite® Plus 488 Anti-Human Phospho-RIPK1 (Ser161) (CL488-66854, Clone:1B2G1) (red), or 0.13 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

---

Applications testées

• Résultats positifs en IF/ICC: cellules HeLa traitées à la calyculine A, cellules U2OS
• Résultats positifs en FC (Intra): cellules HEK-293T traitées à la calyculine A,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66854 cible Phospho-RIPK1 (Ser161) dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: receptor (TNFRSF)-interacting serine-threonine kinase 1
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 74 kDa, 45 kDa
• Numéro d’acquisition GenBank: NM_003804
• Symbole du gène: RIPK1
• Identification du gène (NCBI): 8737
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

RIPK1(Receptor-interacting serine/threonine-protein kinase 1) is primarily involved in mediating TNF-R1-induced cell activation, apoptosis and necroptosis and belongs to a novel class of kinases thatfunction in cell survival and cell death mechanisms(PMID:22685397 ).It has 2 isoforms produced by alternative splicing.It also can exist as the protein with lower molecular weight about 25kd and 45kd in HAEC and HUVEC(PMID:22685397).

{{ptg:RelatedPrimaryAntibodies}}