Anticorps Monoclonal anti-Phospho-S6 Ribosomal protein (Ser235)

Phospho-S6 Ribosomal protein (Ser235) Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(4)

CloneNo.

2A4B6

N° de cat : 67898-1-Ig

Synonymes

P RPS6 Ser235, P RPS6 (Ser235), P RPS6, P S6 Ribosomal protein (Ser235), Phospho-RPS6

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Galerie de données de validation

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  WB analysis using 67898-1-Ig

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control.

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  WB analysis using 67898-1-Ig

  Non-treated and Calyculin A treated cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control.

• 

  WB analysis using 67898-1-Ig

  Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 67898-1-Ig (Phospho-S6 Ribosomal protein (Ser235) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with beta tubulin antibody as loading control.

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  IF Staining of HeLa using 67898-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed untreated and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-S6 Ribosomal protein (Ser235) antibody (67898-1-Ig, Clone: 2A4B6) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  FC experiment of HeLa using 67898-1-Ig

  1x10^6 untreated HeLa cells (dash lines) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.2 μg Phospho-S6 Ribosomal protein (Ser235) Monoclonal antibody (67898-1-Ig, Clone: 2A4B6, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4, blue) was parallel stained as control. Cells were fixed with 4% PFA .

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HEK-293 traitées à la calyculine A, cellules HeLa traitées à la calyculine A, cellules MCF-7 traitées à l'IGF-1, cellules NIH/3T3 traitées à la calyculine A
• Résultats positifs en IF/ICC: 100 nM Calyculin A (30 minutes) treated HeLa cells,
• Résultats positifs en FC (Intra): 100 nM Calyculin A (30 minutes) treated HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

67898-1-Ig cible Phospho-S6 Ribosomal protein (Ser235) dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ribosomal protein S6
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC000524
• Symbole du gène: RPS6
• Identification du gène (NCBI): 6194
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404). 67898-1-Ig specifically recognizes the phosphorylation site of Ser235 or dual site Ser235/236.

Publications

Species	Application	Title
mouse	WB	Sci Immunol; Brain sensing of metabolic state regulates circulating monocytes; Authors - Joao Paulo Cavalcanti de Albuquerque
human	WB	Nat Commun; Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease; Authors - Evangelia Lekka
human	WB	FASEB J; Pharmacological activation of ERβ by arctigenin maintains the integrity of intestinal epithelial barrier in inflammatory bowel diseases.; Authors - Yu Tao
Human		Int J Immunopathol Pharmacol; Sestrin 2 protects human lens epithelial cells from oxidative stress and apoptosis induced by hydrogen peroxide by regulating the mTOR/Nrf2 pathway; Authors - Xiao Tian

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