Anticorps Monoclonal anti-Phospho-S6 Ribosomal protein (Ser235)

Phospho-S6 Ribosomal protein (Ser235) Monoclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2A4B6

N° de cat : CL488-67898

Synonymes

p S6 Ribosomal protein Ser235, p S6 Ribosomal protein (Ser235), p S6 Ribosomal protein, Phospho S6 Ribosomal protein, Phospho S6 Ribosomal protein (Ser235)

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Galerie de données de validation

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  IF Staining of HeLa using CL488-67898

  Immunofluorescent analysis of (4% PFA) fixed Calyculin A treated HeLa cells using CoraLite® Plus 488 Phospho-S6 Ribosomal protein (Ser235) antibody (CL488-67898, Clone: 2A4B6 ) at dilution of 1:200.

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  FC experiment of HeLa using CL488-67898

  1X10^6 HeLa cells untreated (dashed lines) or treated with Calyculin A (red) were intracellularly stained with 0.25 ug CoraLite® Plus 488 Anti-Human Phospho-S6 Ribosomal protein (Ser235) (CL488-67898, Clone:2A4B6), or 0.25 ug Control Antibody (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Applications testées

• Résultats positifs en IF/ICC: cellules HeLa traitées à la calyculine A,
• Résultats positifs en FC (Intra): cellules HeLa traitées à la calyculine A,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-67898 cible Phospho-S6 Ribosomal protein (Ser235) dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ribosomal protein S6
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC000524
• Symbole du gène: RPS6
• Identification du gène (NCBI): 6194
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404). 67898-1-Ig specifically recognizes the phosphorylation site of Ser235 or dual site Ser235/236.

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