Anticorps Polyclonal de lapin anti-Phospho-S6 Ribosomal protein (Ser240/244)

Phospho-S6 Ribosomal protein (Ser240/244) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 29220-1-AP

Synonymes

40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, Phospho-RPS6 (Ser240/244), Phospho-S6 Ribosomal Protein (Ser240/244), ribosomal protein S6, Ribosome Marker, RPS6, S6 ribosomal protein

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Galerie de données de validation

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  WB analysis using 29220-1-AP

  Serum-free treated NIH/3T3 cells and 10 % fetal bovine serum treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29220-1-AP (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:3000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3 traitées au sérum fœtal de bovin à 10 %,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

29220-1-AP cible Phospho-S6 Ribosomal protein (Ser240/244) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ribosomal protein S6
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: NM_001010
• Symbole du gène: RPS6
• Identification du gène (NCBI): 6194
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal protein S6 (RPS6), also named as Phosphoprotein NP33, may play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Ribosomal protein S6 is the major substrate of protein kinases in eukaryote ribosomes. The phosphorylation is stimulated by growth factors, tumor promoting agents, and mitogens and is dephosphorylated at growth arrest.

Publications

Species	Application	Title
human	WB	Biochim Biophys Acta Mol Cell Res; IL-17A's role in exacerbating radiation-induced lung injury: Autophagy impairment via the PP2A-mTOR pathway; Authors - Liangzhong Liu
mouse	WB	Biochimie; Doxorubicin-induced senescence is modulated by the eukaryotic release factor 3a and its polyglycine expansion in HCT116 cells; Authors - Béatrice Jolles
human	WB	Vet Microbiol; Porcine reproductive and respiratory syndrome virus nsp2-related proteins induce host translational arrest by specifically impairing the mTOR signaling cascade; Authors - Xue Jiao

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