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Anticorps Recombinant de lapin anti-Phospho-RPS6KA1 (Thr359/Ser363)

Phospho-RPS6KA1 (Thr359/Ser363) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3O10

N° de cat : CL488-81475

Synonymes

p RPS6KA1, p RPS6KA1 (Thr359/Ser363), p RPS6KA1 Thr359/Ser363, Phospho RPS6KA1, Phospho RPS6KA1 (Thr359/Ser363)

Galerie de données de validation

Filter:
  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated Phospho-RPS6KA1 (Thr (CL488-81475)

    FC experiment of HeLa using CL488-81475

    1X10^6 HeLa cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.25 ug Coralite® Plus 488 Phospho-Rps6ka1 (Thr359/Ser363) Recombinant Antibody (CL488-81475, Clone:3O10)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en FC (Intra)cellules HeLa traitées à la λ phosphatase,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-81475 cible Phospho-RPS6KA1 (Thr359/Ser363) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet ribosomal protein S6 kinase, 90kDa, polypeptide 1
Masse moléculaire calculée 735 aa, 83 kDa
Poids moléculaire observé83-90 kDa
Numéro d’acquisition GenBankBC014966
Symbole du gène RPS6KA1
Identification du gène (NCBI) 6195
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322)

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