Anticorps Recombinant de lapin anti-Phospho-S6 Ribosomal protein (Ser240/244)

Phospho-S6 Ribosomal protein (Ser240/244) Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242048C2

N° de cat : 80575-3-RR

Synonymes

Phos ribosomal protein S6, Phos RPS6, Phos S6 Ribosomal protein (Ser240/244), Phospho RPS6, Phospho-RPS6 (Ser240/244)

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Galerie de données de validation

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  WB analysis using 80575-3-RR

  HEK-293 cells treated with 10% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control.

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  WB analysis of NIH/3T3 using 80575-3-RR

  NIH/3T3 cells treated with 20% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control.

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  WB analysis of PC-12 using 80575-3-RR

  PC-12 cells treated with 20% fetal bovine serum after serum free were subjected to SDS PAGE followed by western blot with 80575-3-RR (Phospho-S6 Ribosomal protein (Ser240/244) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubuliin (66031-1-Ig) antibody as loading control.

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  IF Staining of HepG2 using 80575-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-S6 Ribosomal protein (Ser240/244) antibody (80575-3-RR, Clone: 242048C2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of HEK-293 using 80575-3-RR

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with serum-starved overnight and 10% fetal bovine serum which intracellularly stained with 0.06 ug Phospho-S6 Ribosomal protein (Ser240/244) Recombinant antibody (80575-3-RR, Clone:242048C2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: 20% fetal bovine serum after serum free NIH/3T3 cells, 10% fetal bovine serum after serum free HEK-293 cells, 20% fetal bovine serum after serum free PC-12 cells
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): 10% fetal bovine serum treated HEK-293 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80575-3-RR cible Phospho-S6 Ribosomal protein (Ser240/244) dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ribosomal protein S6
• Masse moléculaire calculée: 29 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC000524
• Symbole du gène: RPS6
• Identification du gène (NCBI): 6194
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ribosomal protein S6 (RPS6) is one of the components of the 40S ribosomal subunit. RPS6 has been functionally regarded as the stimulator and/or inhibitor of certain types of mRNA translation, as well as the regulator of cellular metabolisms, cells size, survival and proliferation. RPS6 is phosphorylated at multiple sites, comprised between Ser235 and Ser247, by the p70 rpS6 kinase (S6K) 1, which is a major downstream effector of the mammalian target of rapamycin complex 1 (mTORC1). Phosphorylation of RPS6 at the dual site Ser235/236 occurs also independently of mTORC1, via the p90 ribosomal S6 kinases (RSK), which are activated by the extracellular signal-regulated kinases (ERK). Recent studies performed in pancreatic β-cells identified PKA as an additional RPS6 kinase, specifically involved in the phosphorylation of Ser235/236. (PMID: 26490682, PMID: 21814187, PMID: 31112404)

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